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mouse methylcellulose complete media without epo  (R&D Systems)


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    R&D Systems mouse methylcellulose complete media without epo
    IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
    Mouse Methylcellulose Complete Media Without Epo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+methylcellulose+complete+media+without+epo/pmc11260449-96-15-22?v=R%26D+Systems
    Average 93 stars, based on 77 article reviews
    mouse methylcellulose complete media without epo - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "IRF2BP2 counteracts the ATF7/JDP2 AP-1 heterodimer to prevent inflammatory overactivation in acute myeloid leukemia (AML) cells"

    Article Title: IRF2BP2 counteracts the ATF7/JDP2 AP-1 heterodimer to prevent inflammatory overactivation in acute myeloid leukemia (AML) cells

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae437

    IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Western Blot, Isolation, Two Tailed Test, Microscopy, Control, Quantitative RT-PCR, RNA Sequencing, Migration



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    R&D Systems mouse methylcellulose complete media without epo
    IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
    Mouse Methylcellulose Complete Media Without Epo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+methylcellulose+complete+media+without+epo/pmc11260449-96-15-22?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    mouse methylcellulose complete media without epo - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier
    mouse methylcellulose complete media  (Bio-Techne corporation)
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    Bio-Techne corporation mouse methylcellulose complete media
    IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
    Mouse Methylcellulose Complete Media, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+methylcellulose+complete+media+without+epo/bio-techne+corporation___hsc007?v=Bio-Techne+corporation
    Average 93 stars, based on 1 article reviews
    mouse methylcellulose complete media - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: Nucleic Acids Research

    Article Title: IRF2BP2 counteracts the ATF7/JDP2 AP-1 heterodimer to prevent inflammatory overactivation in acute myeloid leukemia (AML) cells

    doi: 10.1093/nar/gkae437

    Figure Lengend Snippet: IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: The cells were seeded in duplicates at 5 × 10 3 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3, mIL-6 and mSCF (concentrations as described above), on 35 mm dishes and cultivated at 37°C and 5% CO 2 .

    Techniques: Western Blot, Isolation, Two Tailed Test, Microscopy, Control, Quantitative RT-PCR, RNA Sequencing, Migration